Expression and purification of a synthetic human obese gene product

被引：20

作者：

Altmann, SW ^{[1
]}

Timans, JC ^{[1
]}

Rock, FL ^{[1
]}

Bazan, JF ^{[1
]}

Kastelein, RA ^{[1
]}

机构：

[1] DNAX RES INST MOLEC & CELLULAR BIOL INC,DEPT MOLEC BIOL,PALO ALTO,CA 94304

来源：

PROTEIN EXPRESSION AND PURIFICATION | 1995年 / 6卷 / 06期

关键词：

D O I：

10.1006/prep.1995.0002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Human obese (hOB) protein was recently identified as a secreted hormone-like factor that is exclusively produced by adipose tissue and appears to regulate the size of the body's fat stores. We describe a rapid and efficient repetitive extension PCR method for the construction of a 453-bp synthetic hOB gene with high-frequency codons to optimize expression in Escherichia coli. The use of a bacterial signal sequence fused to hOB together with expression of bacteriocin release protein resulted in efficient release of hOB into the culture medium. The protein was readily purified to homogeneity from the culture medium using a two-step procedure. (C) 1995 Academic Press, Inc.

引用

页码：722 / 726

页数：5

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