IN-VIVO INTERACTION OF RABIES VIRUS PHOSPHOPROTEIN (P) AND NUCLEOPROTEIN (N) - EXISTENCE OF 2 N-BINDING SITES ON P-PROTEIN

被引:95
作者
CHENIK, M [1 ]
CHEBLI, K [1 ]
GAUDIN, Y [1 ]
BLONDEL, D [1 ]
机构
[1] CNRS,GENET VIRUS LAB,F-91198 GIF SUR YVETTE,FRANCE
关键词
D O I
10.1099/0022-1317-75-11-2889
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The rabies virus phosphoprotein (P) and nucleoprotein (N) are involved in transcription and replication of the viral genome. Interaction between N and P was studied in vivo in transfected cells expressing both proteins. Coimmunoprecipitation assays revealed that the N-P complex is present in cells expressing both proteins as well as in infected cells. Furthermore, immunostaining showed that coexpression of N and P was sufficient to induce the formation of cytoplasmic inclusions similar to those found in infected cells. In addition, deletion mutant analysis of P was performed to identify the regions of P interacting with N. The results indicate that at least two independent N-binding sites exist on P protein: one is located in the carboxy-terminal part of the protein and another between amino acids 69 and 177. The formation of cytoplasmic inclusions seems to require the presence of both N-binding sites on P protein.
引用
收藏
页码:2889 / 2896
页数:8
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