THE MAJORITY OF LACTOCOCCAL PLASMIDS CARRY A HIGHLY RELATED REPLICON

被引：80

作者：

SEEGERS, JFML ^{[1
]}

BRON, S ^{[1
]}

FRANKE, CM ^{[1
]}

VENEMA, G ^{[1
]}

KIEWIET, R ^{[1
]}

机构：

[1] CTR BIOL SCI,DEPT GENET,9715 NN HAREN,NETHERLANDS

来源：

MICROBIOLOGY-UK | 1994年 / 140卷

关键词：

LACTOCOCCAL PLASMIDS; PLASMID REPLICATION; PLASMID INCOMPATIBILITY;

D O I：

10.1099/00221287-140-6-1291

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNA sequence analysis and Southern hybridizations, together with complementation experiments, were used to study relationships between lactococcal plasmid replicons. pWVO2, pWVO4 and pWVO5, which co-exist in Lactococcus lactis subsp. cremoris Wg2, and pIL7 (isolated from another strain) all contained a functional replication region which appeared to be very similar to that of some known lactococcal plasmids. They contain a gene encoding a highly conserved RepB protein (60-80% amino acid identity between pWVO2, pWVO4 and pWVO5), which is essential for replication. When supplied in trans, repB of pWVO2 complemented a repB deficiency of pWVO5 Upstream of the repB gene, all these plasmids contain a strongly conserved region including a 22 bp sequence tandemly repeated three-and-a-half times, and an AIT-rich region. The similarity with pWVO2, which is known to replicate via a theta mechanism, suggests that all plasmids of this family are capable of theta replication. Southern hybridizations revealed that many lactococcal strains contain plasmids of this family.

引用

页码：1291 / 1300

页数：10

共 30 条

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