Pharmacological studies on mechanisms involved in Ca2+ oscillations in rat megakaryocytes

被引：3

作者：

Uneyama, C ^{[1
]}

Uneyama, H ^{[1
]}

Takahashi, M ^{[1
]}

Akaike, N ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC MED,DEPT BIOPLAST,FUKUOKA 812,JAPAN

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY-MOLECULAR PHARMACOLOGY SECTION | 1995年 / 291卷 / 03期

关键词：

Ca2+-ATPase; protein kinase C; protein kinase A; calmodulin; megakaryocyte; rat; patch-clamp;

D O I：

10.1016/0922-4106(95)90080-2

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Extracellular application of ATP evoked the oscillatory K+ currents (I-KCa) reflecting oscillation in cytoplasmic Ca2+ concentration ([Ca2+](i)) of megakaryocyte isolated from rat bone marrow. We have reported that the [Ca2+](i) oscillation was regulated by intracellular Ca2+-pumping activity (Uneyama, H., C. Uneyama and N. Akaike, 1993, J. Biol. Chem. 268, 168). Here we found that the Ca2+ pump of the megakaryocyte could be divided into at least two classes according to the sensitivity to phosphorylation-modulating drugs. The effects of protein kinase C and cyclic AMP-dependent protein kinase are complementary, and the effect of Ca2+/calmodulin is independent of the above two kinases. In addition, this is the first report concerning the physiological regulation of Ca2+-ATPase in living cells.

引用

页码：381 / 386

页数：6

共 22 条

[1] EXISTENCE OF ROLIPRAM-SENSITIVE PHOSPHODIESTERASE IN RAT MEGAKARYOCYTE [J].