IDENTIFICATION, NUCLEAR-LOCALIZATION, AND DNA-BINDING ACTIVITY OF THE ZINC FINGER PROTEIN ENCODED BY THE EVI-1 MYELOID TRANSFORMING GENE

被引：77

作者：

MATSUGI, T

MORISHITA, K

IHLE, JN

机构：

[1] ST JUDE CHILDRENS RES HOSP, DEPT BIOCHEM, 332 N LAUDERDALE, MEMPHIS, TN 38105 USA

[2] UNIV TENNESSEE, CTR HLTH SCI, DEPT BIOCHEM, MEMPHIS, TN 38163 USA

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 03期

关键词：

D O I：

10.1128/MCB.10.3.1259

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Activation of the Evi-1 zinc finger gene is a common event associated with transformation of murine myeloid leukemias. To characterize the gene product, we developed antisera against various protein domains. These antisera primarily detected a 145-kilodalton nuclear protein that bound double-stranded DNA. Binding was inhibited by chelating agents and partially restored by zinc ions.

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页码：1259 / 1264

页数：6

相关论文

共 24 条

[1] ONCOGENE JUN ENCODES A SEQUENCE-SPECIFIC TRANS-ACTIVATOR SIMILAR TO AP-1 [J].

ANGEL, P ;

ALLEGRETTO, EA ;

OKINO, ST ;

HATTORI, K ;

BOYLE, WJ ;

HUNTER, T ;

KARIN, M .

NATURE, 1988, 332 (6160) :166-171

[2]

BARTHOLOMEW C, 1989, ONCOGENE, V4, P529

[3] POTENTIAL METAL-BINDING DOMAINS IN NUCLEIC-ACID BINDING-PROTEINS [J].

BERG, JM .

SCIENCE, 1986, 232 (4749) :485-487

[4] FREQUENT INVOLVEMENT OF THE FIM-3-REGION IN FRIEND MURINE LEUKEMIA VIRUS-INDUCED MOUSE MYELOBLASTIC LEUKEMIAS [J].

BORDEREAUX, D ;

FICHELSON, S ;

SOLA, B ;

TAMBOURIN, PE ;

GISSELBRECHT, S .

JOURNAL OF VIROLOGY, 1987, 61 (12) :4043-4045

[5] GROWTH OF FACTOR-DEPENDENT HEMATOPOIETIC PRECURSOR CELL-LINES [J].

DEXTER, TM ;

GARLAND, J ;

SCOTT, D ;

SCOLNICK, E ;

METCALF, D .

JOURNAL OF EXPERIMENTAL MEDICINE, 1980, 152 (04) :1036-1047

[6] STUDIES ON THE INTERACTION OF THE HUMAN C-MYC PROTEIN WITH CELL-NUCLEI - P62C-MYC AS A MEMBER OF A DISCRETE SUBSET OF NUCLEAR PROTEINS [J].

EVAN, GI ;

HANCOCK, DC .

CELL, 1985, 43 (01) :253-261

[7] METAL-DEPENDENT FOLDING OF A SINGLE ZINC FINGER FROM TRANSCRIPTION FACTOR-IIIA [J].

FRANKEL, AD ;

BERG, JM ;

PABO, CO .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (14) :4841-4845

[8] CORRELATION OF CELL-SURFACE PHENOTYPE WITH THE ESTABLISHMENT OF INTERLEUKIN 3-DEPENDENT CELL-LINES FROM WILD-MOUSE MURINE LEUKEMIA VIRUS-INDUCED NEOPLASMS [J].

HOLMES, KL ;

PALASZYNSKI, E ;

FREDRICKSON, TN ;

MORSE, HC ;

IHLE, JN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (19) :6687-6691

[9]

Ihle JN, 1984, ADV VIRAL ONCOL, P95

[10]

IHLE JN, 1989, INT J CELL CLONING, V1, P1