Application of response surface methodology to understand the interaction of media components during pullulan production by Aureobasidium pullulans RBF-4A3

被引：26

作者：

Choudhury, A. Roy ^{[1
]}

Bhattacharyya, M. S. ^{[1
]}

Prasad, G. S. ^{[2
]}

机构：

[1] CSIR, CSIR Inst Microbial Technol IMTECH, BERPDC, Sect 39A, Chandigarh 160036, India

[2] CSIR, CSIR Inst Microbial Technol IMTECH, Microbial Type Culture Collect & Gene Bank MTCC, Chandigarh 160036, India

来源：

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY | 2012年 / 1卷 / 03期

关键词：

Exopolysaccharide; Pullulan; Aureobasidium pullulans; Central composite design; Response surface methodology; Effect of nitrogen sources;

D O I：

10.1016/j.bcab.2012.02.003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Response surface methodology was applied to understand the interactions amongst the components of a complex medium for pullulan production by a novel Aureobasidium pullulans strain using a second order quadratic model. A total of 20 experimental runs were carried out and three dimensional response surfaces were generated to study the interaction. Results indicated that the concentration of yeast extract plays a critical role and has significant influence in pullulan production compared with peptone. Understanding the effect of complex nitrogen sources in the media using statistical methods has resulted in 70.43 g/L pullulan production which is higher as compared to earlier published reports. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：232 / 237

页数：6

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