IDENTIFICATION OF INDIVIDUAL BENZO[C]PHENANTHRENE DIHYDRODIOL EPOXIDE-DNA ADDUCTS BY THE P-32 POSTLABELING ASSAY

被引：21

作者：

CANELLA, KA ^{[1
]}

PELTONEN, K ^{[1
]}

YAGI, H ^{[1
]}

JERINA, DM ^{[1
]}

DIPPLE, A ^{[1
]}

机构：

[1] NIDDKD,BIOORGAN CHEM LAB,BETHESDA,MD 20892

来源：

CHEMICAL RESEARCH IN TOXICOLOGY | 1992年 / 5卷 / 05期

关键词：

D O I：

10.1021/tx00029a015

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Purine deoxyribonucleoside 3'-phosphates were reacted separately with the four configurational isomers of benzo[c]phenanthrene 3,4-dihydrodiol 1,2-epoxide. Products resulting from the cis and trans opening of the epoxide ring by the exocyclic amino groups of deoxyadenosine and deoxyguanosine 3'-phosphates were separated by high-pressure liquid chromatography and identified by comparison of the observed circular dichroism spectra with the known spectra for the corresponding nucleoside adducts. The 16 structurally identified benzo[c]phenanthrene-purine deoxyribonucleoside 3'-phosphate adducts were then separately postlabeled according to the Randerath method, and the positions of the individual bisphosphates were mapped by thin-layer chromatography. Chromatographic conditions were developed that allowed separation of the four adducts for 3 of the 4 dihydrodiol epoxide isomers.

引用

页码：685 / 690

页数：6

共 15 条

[11] NUCLEASE-P1-MEDIATED ENHANCEMENT OF SENSITIVITY OF P-32 POSTLABELING TEST FOR STRUCTURALLY DIVERSE DNA ADDUCTS [J].