ACTION OF RAT-LIVER GAL-BETA-1-4GLCNAC ALPHA(2-6)-SIALYLTRANSFERASE ON MAN-BETA-1-4GLCNAC-BETA-OME, GALNAC-BETA-1-4GLCNAC-BETA-OME, GLC-BETA-1-4GLCNAC-BETA-OME AND GLCNAC-BETA-1-4GLCNAC-BETA-OME AS SYNTHETIC SUBSTRATES

被引：19

作者：

HOKKE, CH ^{[1
]}

VANDERVEN, JGM ^{[1
]}

KAMERLING, JP ^{[1
]}

VLIEGENTHART, JFG ^{[1
]}

机构：

[1] UNIV UTRECHT,BIJVOET CTR,DEPT BIOORGAN CHEM,POB 80075,3508 TB UTRECHT,NETHERLANDS

来源：

GLYCOCONJUGATE JOURNAL | 1993年 / 10卷 / 01期

关键词：

ALPHA(2-6)-SIALYLTRANSFERASE; CMP-NEU5AC; GAL-BETA-1-4GLCNAC ALPHA(2-6)-N-ACETYLNEURAMINYLTRANSFERASE; SIALYLOLIGOSACCHARIDES; H-1-NMR SPECTROSCOPY;

D O I：

10.1007/BF00731191

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of synthetic Manbeta1-4GlcNAcbeta-OMe, GalNAcbeta1-4GlcNAcbeta-OMe, Glcbeta1-4GlcNAcbeta-OMe, and GlcNAcbeta1-4GlcNAcbeta-OMe with CMP-Neu5Ac and rat liver Galbeta1-4GlcNAc alpha(2-6)-sialyltransferase resulted in the formation of Neu5Acalpha2-6Manbeta1-4GlcNAcbeta-OMe, Neu5Acalpha2-6GalNAcbeta1-4GlcNAcbeta-OMe, Neu5Acalpha2-6Glcbeta1-4GlcNAcbeta-OMe and Neu5Acalpha2-6GlcNAcbeta1-4GlcNAcbeta-OMe, respectively. Under conditions which led to quantitative conversion of Galbeta1-4GlcNAcbeta-OEt into Neu5Acalpha2-6Galbeta1-4GlcNAcbeta-OEt, the aforementioned products were obtained in yields of 4%, 48%, 16% and 8%, respectively. HPLC on Partisil 10 SAX was used to isolate the various sialyltrisaccharides, and identification was carried out using 1- and 2-dimensional 500-MHz H-1-NMR spectroscopy.

引用

页码：82 / 90

页数：9

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