PURIFICATION OF 2 TRANSCRIPTION FACTORS REQUIRED FOR INITIATION BY MAMMALIAN RNA POLYMERASE-II

被引：7
作者：
KAWAGUCHI, T
KITAJIMA, S
NIHO, Y
ODA, T
GERMINO, J
WEISSMAN, SM
YASUKOCHI, Y
机构：
[1] KYORITSU COLL PHARMACEUT SCI,DEPT BIOCHEM,MINATO KU,TOKYO 105,JAPAN
[2] KYUSHU UNIV,FAC MED,DEPT INTERNAL MED 1,HIGASHI KU,FUKUOKA 812,JAPAN
[3] YALE UNIV,SCH MED,DEPT HUMAN GENET,NEW HAVEN,CT 06510
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 17期
关键词：
D O I：
10.1073/pnas.87.17.6619
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have purified two general transcription factors (FA and FE) necessary for specific transcription by mammalian RNA polymerase II to near homogeneity. Both activities are associated with peptides of ≈33 kDa. FA and FE do not replace one another and show different kinetics of action in a sarkosyl block assay. In particular, FE participated in a rapid reaction after the formation of an initial complex with the other transcription factors. Furthermore, FE can associate with purified calf thymus RNA polymerase II.
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页码：6619 / 6623
页数：5
相关论文
共 39 条
[1]   THE HUMAN C-MYC-ONCOGENE - STRUCTURAL CONSEQUENCES OF TRANSLOCATION INTO THE IGH LOCUS IN BURKITT-LYMPHOMA [J].
BATTEY, J ;
MOULDING, C ;
TAUB, R ;
MURPHY, W ;
STEWART, T ;
POTTER, H ;
LENOIR, G ;
LEDER, P .
CELL, 1983, 34 (03) :779-787
[2]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[3]   5 INTERMEDIATE COMPLEXES IN TRANSCRIPTION INITIATION BY RNA POLYMERASE-II [J].
BURATOWSKI, S ;
HAHN, S ;
GUARENTE, L ;
SHARP, PA .
CELL, 1989, 56 (04) :549-561
[4]   RAP30/74 - A GENERAL INITIATION-FACTOR THAT BINDS TO RNA POLYMERASE-II [J].
BURTON, ZF ;
KILLEEN, M ;
SOPTA, M ;
ORTOLAN, LG ;
GREENBLATT, J .
MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (04) :1602-1613
[5]   AN RNA POLYMERASE-II TRANSCRIPTION FACTOR BINDS TO AN UPSTREAM ELEMENT IN THE ADENOVIRUS MAJOR LATE PROMOTER [J].
CARTHEW, RW ;
CHODOSH, LA ;
SHARP, PA .
CELL, 1985, 43 (02) :439-448
[6]  
COHEN RB, 1988, J BIOL CHEM, V263, P10377
[7]   FORMATION OF STABLE PRE-INITIATION COMPLEXES BETWEEN EUKARYOTIC CLASS-B TRANSCRIPTION FACTORS AND PROMOTER SEQUENCES [J].
DAVISON, BL ;
EGLY, JM ;
MULVIHILL, ER ;
CHAMBON, P .
NATURE, 1983, 301 (5902) :680-686
[8]  
DIGNAM JD, 1983, METHOD ENZYMOL, V101, P582
[9]  
FIRE A, 1984, J BIOL CHEM, V259, P2509
[10]  
FLORES O, 1989, J BIOL CHEM, V264, P8913
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