STRUCTURAL WATER IN OXIDIZED AND REDUCED HORSE HEART CYTOCHROME-C

被引：58

作者：

QI, PX ^{[1
]}

URBAUER, JL ^{[1
]}

FUENTES, EJ ^{[1
]}

LEOPOLD, MF ^{[1
]}

WAND, AJ ^{[1
]}

机构：

[1] UNIV ILLINOIS,DEPT BIOCHEM,URBANA,IL 61801

来源：

NATURE STRUCTURAL BIOLOGY | 1994年 / 1卷 / 06期

关键词：

D O I：

10.1038/nsb0694-378

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The existence of structural water in the interior of both oxidized and reduced horse-heart cytochrome c in solution is demonstrated using nuclear magnetic resonance spectroscopy. Six water molecules have been located in ferrocytochrome c and five in ferricytochrome c, with residence times greater than a few hundred picoseconds. Two water molecules are located in the haem crevice, one of which is found to undergo a large change in position with a change of oxidation state. Both of these observations indicate that buried structural waters in the haem crevice have, by microscopic dielectric effects, significant roles in the setting of the solvent reorganization energy associated with electron transfer.

引用

页码：378 / 382

页数：5

共 41 条

[1] PRACTICAL ASPECTS OF TWO-DIMENSIONAL TRANSVERSE NOE SPECTROSCOPY [J].