IMAGING BY FLUORESCENCE VIDEOMICROSCOPY OF INDIVIDUAL SINGLE-STRANDED-DNA MOLECULES IN SOLUTION

被引：0
作者：
AUZANNEAU, I
BARREAU, C
SALOME, L
机构：
[1] CNRS,CTR RECH PAUL PASCAL,AV A SCHWEITZER,F-33600 PESSAC,FRANCE
[2] UNIV BORDEAUX 2,GENET LAB,CNRS,URA 542,F-33402 TALENCE,FRANCE
[3] SOC LETRAGE,F-93160 NOISY LE GRAND,FRANCE
来源：
COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES | 1993年 / 316卷 / 05期
关键词：
SINGLE STRANDED DNA; FLUORESCENCE MICROSCOPY; ASYMMETRIC CYANINE DYE;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We demonstrate that the observation by fluorescence video-microscopy of individual single stranded DNA molecules is now possible owing to the use of an asymmetric cyanine (YOYO-1) belonging to a new class of fluorescent dyes complexing the DNA. The fluorescence of labelled DNA persists in denaturing conditions showing that the dye does not attach simply to the possible secondary structures. Furthermore it is stable under electrophoresis and enables the imaging of stretched molecules.
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页码：459 / 462
页数：4
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