RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISMS IN CHLOROPLAST DNA FROM 6 SPECIES OF EUCALYPTUS

被引:16
作者
STEANE, DA
WEST, AK
POTTS, BM
OVENDEN, JR
REID, JB
机构
[1] UNIV TASMANIA, DEPT BIOCHEM, HOBART, TAS 7001, AUSTRALIA
[2] UNIV TASMANIA, DEPT ZOOL, HOBART, TAS 7001, AUSTRALIA
基金
澳大利亚研究理事会;
关键词
D O I
10.1071/BT9910399
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Chloroplast DNA was extracted from six species of Eucalyptus (E. perriniana, E. nitens, E. ovata, E. regnans, E. amygdalina and E. risdonii). Digests with four restriction enzymes (Hind III, Xho I, Nco I and Eco RV) revealed restriction fragment length polymorphisms (RFLPs) between subgenera, between species and within species. However, no variation in fragment pattern was detected with Sac II or Pst I. The subgenera Monocalyptus and Symphyomyrtus were clearly differentiated by their RFLP patterns where, with the exception of one outlying specimen of E. amygdalina, 45% of all polymorphic fragments were specific to one or other subgenus. While species from different subgenera and series were well differentiated it was more difficult to differentiate species within series with the low sample sizes used. However, the average net divergence between species increased with increasing taxonomic distance between species, from 0.02% within series, 0.20% between species from different series within subgenera, to 0.99% of nucleotides per nucleotide site for species from different subgenera. These results would suggest that cpDNA may provide a valuable new tool for phylogenetic analyses within the genus. Based on Eco RV digests the eucalypt chloroplast genome was estimated at 143 kb.
引用
收藏
页码:399 / 414
页数:16
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