ANTHOCYANIN RELEASE FROM GRAPE (VITIS-VINIFERA L) CELL-SUSPENSION

In order to develop a protocol for the release of intravacuolarly-stored anthocyanins from grapevine (Vitis vinifera L.) cell suspension with preserved viability, the effects of extracellular pH modifications, of solid adsorbents in the culture medium (two-phase system) and of chemical permeabilization were investigated. The solid adsorbent Amberlite IR-120 and chemical permeabilization were efficient in enabling the release of up to 70-80% of vacuolar anthocyanins. However, the release was always at the expense of a proportionate loss in cell viability. Results demonstrate the irreversible nature of anthocyanin sequestration.