APPLICATION OF POLYMYXIN-COATED POLYESTER CLOTH TO THE SEMIQUANTITATION OF SALMONELLA IN PROCESSED FOODS

被引：19

作者：

BLAIS, BW

YAMAZAKI, H

机构：

[1] CARLETON UNIV,DEPT BIOL,OTTAWA K1S 5B6,ONTARIO,CANADA

[2] CARLETON UNIV,INST BIOCHEM,OTTAWA K1S 5B6,ONTARIO,CANADA

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1991年 / 14卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

FOOD MANUFACTURING; MONITORING; SALMONELLA; POLYMYXIN;

D O I：

10.1016/0168-1605(91)90035-N

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A rapid and economical semi-quantitative test for Salmonella cells in foods is proposed. Food samples containing different levels of Salmonella cells were homogenized and serially diluted in enrichment broths and then incubated for about 20 h at 37-degrees-C. The presence of Salmonella cells in each dilution was assayed by capturing deoxycholate-extracted Salmonella lipopolysaccharides on a sheet of polymyxin-coated polyester cloth, followed by colorimetric detection with an anti-Salmonella antibody-enzyme conjugate. The minimum dilutions which resulted in no detectable growth were correlated with the extent of Salmollella contamination in the food samples.

引用

页码：43 / 49

页数：7