AFFINITY CHROMATOGRAPHY OF ALPHA-AMYLASES ON CROSS-LINKED STARCH

被引：0

作者：

WEBER, M ^{[1
]}

FOGLIETTI, MJ ^{[1
]}

PERCHERON, F ^{[1
]}

机构：

[1] UNIV PARIS 5, FAC SCI PHARM & BIOL,CNRS,CHIM BIOL LAB, EQUIPE RECH 99, F-75006 PARIS, FRANCE

来源：

BIOCHIMIE | 1976年 / 58卷 / 11-1期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Affinity chromatography on cross-linked starch affords a simple and rapid procedure for .alpha.-amylases (EC 3.2.1.1.) purification. When starch is cross-linked in alkaline medium by epichlorhydrin in the conditions described, the insoluble polysaccharide obtained is able to retain specifically the .alpha.-amylase which is then eluted with 2 M maltose solution. .alpha.-Amylase can be obtained in a pure form with a 60% yield. The exoenzyme .beta.-amylase (EC 3.2.1.2) is not retained by the support and is eluted with other contaminant proteins. This procedure allows the separation of the endo- and exoamylase activities.

引用

页码：1299 / 1302

页数：4

共 10 条

[1] AFFINITY COLUMN PURIFICATION OF AMYLASES ON PROTEIN INHIBITORS FROM WHEAT KERNEL [J].

BUONOCORE, V ;

POERIO, E ;

GRAMENZI, F ;

SILANO, V .

JOURNAL OF CHROMATOGRAPHY, 1975, 114 (01) :109-114

[2] PURIFICATION OF POLYGALACTURONASE FROM HELIX-POMATIA AFFINITY CHROMATOGRAPHY ON ITS OWN CROSS-LINKED SUBSTRATE [J].

FOGLIETTI, MJ ;

GIRERD, H ;

PERCHERON, F .

BIOCHIMIE, 1975, 57 (05) :667-668

[3] STUDY OF CROSSLINKING REACTION BETWEEN EPICHLOROHYDRIN AND STARCH [J].

KUNIAK, L ;

MARCHESSAULT, RH .

STARKE, 1972, 24 (04) :110-+

[4] USE OF CROSS-LINKED AMYLOSE IN ESTABLISHING ALPHA-AMYLASE OR BETA-AMYLASE ACTIVITY OF AN AMYLOLYTIC PREPARATION [J].

MATEESCU, MA ;

SCHELL, HD ;

MIHAILESCU, F ;

CORNOIU, I .

BIOCHIMIE, 1976, 58 (07) :875-877

[5]

Nelson N, 1944, J BIOL CHEM, V153, P375

[6] SELECTIVE PURIFICATION OF ASPERGILLUS-NIGER ENDOPOLYGALACTURONASE BY AFFINITY CHROMATOGRAPHY ON CROSSLINKED PECTIC ACID [J].

REXOVABENKOVA, L ;

TIBENSKY, V .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 268 (01) :187-+

[7]

SCHELL HD, UNPUBLISHED RESULTS

[8]

SOMOGYI M, 1945, J BIOL CHEM, V160, P61

[9]

STREET HV, 1963, METHOD ENZYMAT AN, P854

[10] COMPETITIVE AFFINITY CHROMATOGRAPHY OF WHEAT ALPHA-AMYLASE [J].

TKACHUK, R .

FEBS LETTERS, 1975, 52 (01) :66-68

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