Nongravid females of Heterobilharzia americana were found in mesenteric blood vessels from 3 of 40 (7.5%) white-tailed deer (Odocoileus virginianus) examined at necropsy during October and November 1990 in southern Florida. Ah infected deer were females ranging in age from 2 to 5.5 yr. This represents the first report of this schistosome from white tailed deer in Florida and the second report from this host in North America.