SEQUENCE-SPECIFIC BINDING OF TRANSFER-RNA BY GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE

被引：425
作者：
SINGH, R
GREEN, MR
机构：
[1] Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester
来源：
SCIENCE | 1993年 / 259卷 / 5093期
关键词：
D O I：
10.1126/science.8420004
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A transfer RNA (tRNA) binding protein present in HeLa cell nuclear extracts was purified and identified as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Studies with mutant tRNAs indicated that GAPDH recognizes both sequence and structural features in the RNA. GAPDH discriminated between wild-type tRNA and two tRNA mutants that are defective in nuclear export, which suggests that the protein may participate in RNA export. The cofactor nicotinamide adenine dinucleotide disrupted complex formation between tRNA and GAPDH and thus may share a common binding site with the RNA. Indirect immunofluorescence experiments showed that GAPDH is present in the nucleus as well as in the cytoplasm.
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页码：365 / 368
页数：4
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