FLOW CYTOMETRIC AND MOLECULAR ANALYSIS OF PROLIFERATIVE ACTIVITY AND DNA CONTENT IN NEUROBLASTOMA - PRESENCE OF STATIONARY CELLS IN S-PHASE

被引：0

作者：

DOMINICI, C

NEGRONI, A

ROMEO, A

MCDOWELL, H

PADULA, A

PUCCI, S

CAPPELLI, C

CASTELLO, MA

RASCHELLA, G

机构：

[1] INST GUSTAVE ROUSSY,CLIN & MOLEC PHARMACOL LAB,F-94805 VILLEJUIF,FRANCE

[2] ENTE NATL ENERGIA ATOM CASACCIA,CTR RIC ENERGIA,DIV MOLEC BIOL,I-00060 ROME,ITALY

来源：

ANTICANCER RESEARCH | 1992年 / 12卷 / 01期

关键词：

NEUROBLASTOMA; CYTOKINETICS; DNA CONTENT; N-MYC; HISTONE-H3;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Twenty-nine previously untreated neuroblastomas were analyzed for DNA content and percentage of cells in S-phase, both determined by flow cytometry, and N-myc oncogene copy number. Twelve of them were also tested for histone H3 transcript levels as a marker of actual proliferative activity. A significantly higher S-phase fraction was associated with advanced stages of disease, unfavorable (i.e., near-diploid and near-tetraploid) DNA content, and N-myc amplification. The occurrence of six tumors with a remarkable (greater-than-or-equal-to 10%) S-phase fraction but lacking histone H3 transcripts suggests the presence of stationary S-phase cells in neuroblastoma.

引用

页码：59 / 63

页数：5

共 7 条

[1] FLOW CYTOMETRIC (DNA CONTENT AND S-PHASE FRACTION) ANALYSIS OF BREAST-CANCER
BATSAKIS, JG
SNEIGE, N
ELNAGGAR, AK
CANCER, 1993, 71 (06) : 2151 - 2153
[2] Endometrial carcinomas - Flow cytometric DNA content and S-phase values
Jacobsen, M
Jakobsen, A
Nedergaard, L
Andersen, JE
Nielsen, K
PATHOLOGY RESEARCH AND PRACTICE, 1997, 193 (04) : 283 - 290
[3] N-myc and DNA content in neuroblastoma: A biparametric flow cytometric analysis
Chassevent, A
Benard, J
BULLETIN DU CANCER, 1997, 84 (03) : 229 - 234
[4] Quality control study by the French cytometry association on flow cytometric DNA content and S-phase fraction (S%)
Dhautcourt, JL
Spyratos, F
Chassevent, A
CYTOMETRY, 1996, 26 (01): : 32 - 39
[5] Flow cytometric analysis of the DNA content of bovine and human bone marrow cells
Hoeben, D
Burvenich, C
Lenjou, M
Nijs, G
Massart-Leën, AM
Van Bockstaele, D
VETERINARY QUARTERLY, 2000, 22 (02) : 117 - 120
[6] Flow cytometric DNA content analysis of neoplastic cells in haemolymph of the cockle Cerastoderma edule
da Silva, PM
Soudant, P
Carballal, MJ
Lambert, C
Villalba, A
DISEASES OF AQUATIC ORGANISMS, 2005, 67 (1-2) : 133 - 139
[7] PROGNOSTIC-SIGNIFICANCE OF CELLS WITH A HIGH DNA CONTENT AND S-PHASE FRACTION IN LYMPH-NODE NEGATIVE BREAST-CANCER
WAGNER, T
ADLER, A
KUBISTA, E
WIENER KLINISCHE WOCHENSCHRIFT, 1993, 105 (24) : 712 - 714

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