ESTABLISHMENT OF A GERM-LINE COMPETENT C57BL/6 EMBRYONIC STEM-CELL LINE

被引：131

作者：

LEDERMANN, B

BURKI, K

机构：

[1] Preclinical Research, Sandoz Pharma Ltd.

来源：

EXPERIMENTAL CELL RESEARCH | 1991年 / 197卷 / 02期

关键词：

D O I：

10.1016/0014-4827(91)90430-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of the male ES cell lines tested (BL/6-III) was shown to be karyotypically stable and germ-line competent when introduced into BALB/c host blastocysts. These results demonstrate that ES cell lines from inbred mouse strains other than 129/Sv may be used as vectors to introduce selected mutations into the germline of mice. © 1991.

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页码：254 / 258

页数：5

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