Circular oligonucleotides. Hybridization with 5S rRNA and digestion of RNA-DNA duplex with RNase H

被引：0

作者：

Fedorova, OA ^{[1
]}

Gottikh, MB ^{[1
]}

Romanova, EA ^{[1
]}

Oretskaya, TS ^{[1
]}

Dolinnaya, NG ^{[1
]}

Shabarova, ZA ^{[1
]}

机构：

[1] MOSCOW MV LOMONOSOV STATE UNIV,DEPT CHEM,MOSCOW 119899,RUSSIA

来源：

MOLECULAR BIOLOGY | 1995年 / 29卷 / 05期

关键词：

circular oligonucleotides; chemical ligation; RNase H;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemical ligation was employed to synthesize circular oligonucleotides containing 1,2-dideoxy-D-ribofuranose inserts or nonmodified ones. Such oligonucleotides are able to form stable DNA duplexes with short oligonucleotides complementary to a part of the circular molecule. Circular oligonucleotides are able to interact with 120-unit long RNA; the hybrid duplexes formed may be attacked by RNase H.

引用

页码：689 / 693

页数：5

共 2 条

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