IDENTIFICATION OF CYTOPHAGA PSYCHROPHILA BY PCR TARGETED 16S-RIBOSOMAL RNA

被引：107

作者：

TOYAMA, T ^{[1
]}

KITATSUKAMOTO, K ^{[1
]}

WAKABAYASHI, H ^{[1
]}

机构：

[1] UNIV TOKYO, OCEAN RES INST, NAKANO KU, TOKYO 164, JAPAN

来源：

FISH PATHOLOGY | 1994年 / 29卷 / 04期

关键词：

D O I：

10.3147/jsfp.29.271

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

On the basis of the 16S rRNA sequence data analysis among the closely related species, the specific primers for Cytophaga psychrophila were constructed. The specificity in amplifying the 16S rRNA of C. psychrophila was confirmed by using some selected strains of the related species. In addition, it was revealed that these specific primers distinguished C. psychrophila from other principal fish pathogens. The present PCR technique is expected to be a powerful tool for the diagnosis of cold-water disease.

引用

页码：271 / 275

页数：5

共 10 条

[1] PHENOTYPIC AND GENOMIC STUDIES OF CYTOPHAGA-PSYCHROPHILA ISOLATED FROM DISEASED RAINBOW-TROUT (ONCORHYNCHUS-MYKISS) IN FRANCE
BERNARDET, JF
KEROUAULT, B
[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1989, 55 (07) : 1796 - 1800
[2] BORG AF, 1960, WILDL DIS, V8, P1
[3] HIGGINS DG, 1992, COMPUT APPL BIOSCI, V8, P189
[4] RAPID AND SENSITIVE PROTEIN SIMILARITY SEARCHES
LIPMAN, DJ
PEARSON, WR
[J]. SCIENCE, 1985, 227 (4693) : 1435 - 1441
[5] PHYLOGENETIC DIVERSITY OF THE GENUS CYTOPHAGA REVEALED BY 16S RIBOSOMAL-RNA SEQUENCING AND MENAQUINONE ANALYSIS
NAKAGAWA, Y
YAMASATO, K
[J]. JOURNAL OF GENERAL MICROBIOLOGY, 1993, 139 : 1155 - 1161
[6] NAKAGAWA Y, 1993, THESIS U TOKYO
[7] NISHIMURA M, 1992, B JAP SOC MICROBIOL, V7, P43
[8] A STUDY ON SEROTYPING OF CYTOPHAGA-PSYCHROPHILA ISOLATED FROM FISHES IN JAPAN
WAKABAYASHI, H
TOYAMA, T
IIDA, T
[J]. FISH PATHOLOGY, 1994, 29 (02): : 101 - 104
[9] WAKABAYASHI H, 1991, Fish Pathology, V26, P211
[10] 16S RIBOSOMAL DNA AMPLIFICATION FOR PHYLOGENETIC STUDY
WEISBURG, WG
BARNS, SM
PELLETIER, DA
LANE, DJ
[J]. JOURNAL OF BACTERIOLOGY, 1991, 173 (02) : 697 - 703

← 1 →