In a series of 385 sera obtained from volunteer blood donors positive for the first-generation hepatitis C virus assay (Ortho), the viral genome was detected by polymerase chain reaction (PCR) in 89 sera (23%). Most PCR-positive sera were found positive with the c100-3 neutralisation assay (Abbott) and by two second-generation enzyme immunoassays (Abbott, Ortho). However overall specificity of these assays was rather low. By immunoblotting (Innogenetics and Chiron/Ortho) the specificity could be considerably improved and the best correlation with carrier state was obtained when analysing the results for lane-specific reaction: all 89 viral carriers and only 9 other donors had antibodies against structural 'core' epitopes. From the present data we can conclude that in screening a volunteer blood donor population the confirmation of antibodies against 'core' epitopes by immunoblotting is strongly associated with viral carriage.
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UNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,VIROL SECT,S-21401 MALMO,SWEDENUNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,VIROL SECT,S-21401 MALMO,SWEDEN
ZHANG, YY
GUO, LS
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UNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,VIROL SECT,S-21401 MALMO,SWEDENUNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,VIROL SECT,S-21401 MALMO,SWEDEN
GUO, LS
HAO, LJ
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UNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,VIROL SECT,S-21401 MALMO,SWEDENUNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,VIROL SECT,S-21401 MALMO,SWEDEN
HAO, LJ
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HANSSON, BG
WIDELL, A
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UNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,VIROL SECT,S-21401 MALMO,SWEDENUNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,VIROL SECT,S-21401 MALMO,SWEDEN
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UNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,CLIN VIROL LAB,VIROL SECT,S-21401 MALMO,SWEDENUNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,CLIN VIROL LAB,VIROL SECT,S-21401 MALMO,SWEDEN
ZHANG, YY
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HANSSON, BG
WIDELL, A
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UNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,CLIN VIROL LAB,VIROL SECT,S-21401 MALMO,SWEDENUNIV LUND,MALMO GEN HOSP,DEPT MED MICROBIOL,CLIN VIROL LAB,VIROL SECT,S-21401 MALMO,SWEDEN