CONFIRMATION OF HEPATITIS-C VIRUS POSITIVE BLOOD-DONORS BY IMMUNOBLOTTING AND POLYMERASE CHAIN-REACTION

被引:0
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作者
DEBEENHOUWER, H [1 ]
VERHAERT, H [1 ]
CLAEYS, H [1 ]
VERMYLEN, C [1 ]
机构
[1] BELGIAN RED CROSS,CTR BLOOD TRANSFUS,LOUVAIN,BELGIUM
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中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In a series of 385 sera obtained from volunteer blood donors positive for the first-generation hepatitis C virus assay (Ortho), the viral genome was detected by polymerase chain reaction (PCR) in 89 sera (23%). Most PCR-positive sera were found positive with the c100-3 neutralisation assay (Abbott) and by two second-generation enzyme immunoassays (Abbott, Ortho). However overall specificity of these assays was rather low. By immunoblotting (Innogenetics and Chiron/Ortho) the specificity could be considerably improved and the best correlation with carrier state was obtained when analysing the results for lane-specific reaction: all 89 viral carriers and only 9 other donors had antibodies against structural 'core' epitopes. From the present data we can conclude that in screening a volunteer blood donor population the confirmation of antibodies against 'core' epitopes by immunoblotting is strongly associated with viral carriage.
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页码:198 / 203
页数:6
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