ISOLATION OF GENES FOR LOW-TEMPERATURE-INDUCED PROTEINS IN RICE BY A SIMPLE SUBTRACTIVE METHOD

被引:0
|
作者
AGUAN, K [1 ]
SUGAWARA, K [1 ]
SUZUKI, N [1 ]
KUSANO, T [1 ]
机构
[1] AKITA PREFECTURAL COLL AGR,INST BIOTECHNOL,PLANT GENET ENGN LAB,OHGATA,AKITA 01004,JAPAN
关键词
ABSCISIC ACID; CDNA SUBTRACTION; LOW TEMPERATURE; ORYZA-SATIVA;
D O I
暂无
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
We have isolated cDNAs that correspond to three distinct low-temperature-induced mRNAs from rice cells using an effective, simple subtraction method. The corresponding genes were designated lip (gene encoding low-temperature-induced protein) -5, -9 and -19. The expression of these genes was slightly stimulated (lip5 and lip19) or unaffected (lip9) by abscisic acid, unlike the expression of the rab16A-D genes which are readily induced by either abscisic acid or high osmotic stress. Time-course analysis revealed that while the lip5 gene was induced 3 h after temperature down-shift, and the lip9 and lip19 genes were induced 6 h after such a shift.
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页码:1285 / 1289
页数:5
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