UPTAKE OF L-LACTATE BY CULTURED RAT-BRAIN NEURONS

被引：111

作者：

DRINGEN, R ^{[1
]}

WIESINGER, H ^{[1
]}

HAMPRECHT, B ^{[1
]}

机构：

[1] UNIV TUBINGEN,INST PHYSIOL CHEM,D-72076 TUBINGEN,GERMANY

来源：

NEUROSCIENCE LETTERS | 1993年 / 163卷 / 01期

关键词：

NEURON; LACTATE; TRANSPORT; ENERGY METABOLISM; MONOCARBOXYLATE CARRIER;

D O I：

10.1016/0304-3940(93)90215-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The uptake of L-lactate was investigated in neuronal primary cultures derived from embryonic rat brain with a radioactive tracer method. After preincubation of the cells in glucose-free buffer for 30 min, uptake increased with time for at least IO min. A saturable component of uptake was found with half-maximal uptake at 10 mM lactate. This saturable component was abolished in the presence of 10 mM alpha-cyano-4-hydroxycinnamic acid. In addition, a non-saturable component dominated the uptake at high concentrations of lactate. Uptake was accelerated with decreasing pH, and was inhibited considerably by pyruvate. It is concluded that neurons are endowed with a lactate transport system which resembles in its properties the monocarboxylate carrier of peripheral tissues.

引用

页码：5 / 7

页数：3

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