TRANSFORMING GROWTH FACTOR-BETA-1 IS A POTENT SURVIVAL FACTOR FOR RAT EMBRYO MOTONEURONS IN CULTURE

被引：132
|
作者：
MARTINOU, JC [1 ]
LEVANTHAI, A [1 ]
VALETTE, A [1 ]
WEBER, MJ [1 ]
机构：
[1] CNRS, PHARMACOL & TOXICOL FONDAMENTALES LAB, F-31077 TOULOUSE, FRANCE
来源：
DEVELOPMENTAL BRAIN RESEARCH | 1990年 / 52卷 / 1-2期
关键词：
Cell sorting; Choline acetyltransferase; Motoneuron; Retrograde labelling; Survival factor; Transforming growth factor;
D O I：
10.1016/0165-3806(90)90233-O
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have studied the effects of transforming growth factor β1 (TGFβ1) on the survival of embryonic motoneurons in culture. For this purpose, E14 rat embryo motoneurons were purified to more than 90% homogeneity by cell sorting and cultured at low density on monolayers of cortex astrocytes. Subnanomolar concentrations of TGFβ1 (40-500 pM) increased the survival of motoneurons 2-fold after 9-11 days in culture. The increase in choline acetyltransferase (ChAT) activity per culture caused by TGFβ1 was attributable to its effects on survival. Comparable results were found with motoneurons cultured on lysed astrocytes, suggesting that the effects of the factor are not mediated by non-neuronal cells, but that motoneurons are a target for TGFβ1. © 1990.
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收藏
页码：175 / 181
页数：7
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