IMMUNOLOGY AND MORPHOLOGY STUDIES ON THE PROLIFERATION OF IN-VITRO CULTIVATED ECHINOCOCCUS-MULTILOCULARIS METACESTODES

被引：98
作者：
HEMPHILL, A
GOTTSTEIN, B
机构：
[1] UNIV BERN,FAC VET MED,INST PARASITOL,CH-3001 BERN,SWITZERLAND
[2] UNIV BERN,FAC MED,CH-3001 BERN,SWITZERLAND
来源：
PARASITOLOGY RESEARCH | 1995年 / 81卷 / 07期
关键词：
D O I：
10.1007/BF00932028
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
The larval stage of Echinococcus multilocularis causes alveolar echinococcosis (AE) in various mammals, including humans. Traditionally metacestodes are maintained in the laboratory by serial transplantation passages into susceptible animals such as mice or gerbils. However, in animal models it has always been difficult to draw definite conclusions about the factors modulating metacestode differentiation, and investigations on gene expression and respective regulation have been hampered by the complexicity of the host-parasite interplay. This paper describes the maintenance and proliferation of E. multilocularis metacestodes as well as the formation of protoscolices in a chemically defined medium devoid of host influence. The interactive role of a heterologous human cell line (CACO2) in the in vitro development of metacestodes was also assessed. The morphology and ultrastructure of in vitro-generated metacestodes was studied using scanning (SEM) and transmission electron microscopy (TEM). Different cultivation procedures were analyzed in terms of expression of Band T-cell epitopes and of the relevant laminated layer-antigen Em2; the exact localization of this antigen was further demonstrated by immunogold electron microscopy.
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页码：605 / 614
页数：10
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