ELECTRON-MICROSCOPIC EXAMINATION OF THE EXTRACELLULAR POLYMERIC SUBSTANCES IN ANAEROBIC GRANULAR BIOFILMS

被引：17
作者：
MACLEOD, FA
GUIOT, SR
COSTERTON, JW
机构：
[1] NATL RES COUNCIL CANADA,BIOTECHNOL RES INST,MONTREAL,PQ H4P 2R2,CANADA
[2] NAT RESOURCES CANADA,CTR MINERAL & ENERGY TECHNOL,OTTAWA,ON K1A 0G1,CANADA
[3] MONTANA STATE UNIV,CTR BIOFILM ENGN,BOZEMAN,MT 59717
来源：
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 1995年 / 11卷 / 05期
关键词：
ANAEROBIC GRANULE; ELECTRON MICROSCOPY; EXTRACELLULAR POLYMERIC SUBSTANCES;
D O I：
10.1007/BF00286356
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Scanning electron microscopy revealed that collapsed extracellular polymeric substances (EPS) surrounded bacteria present in granular sludge. Treatment of granular sludge with whole-cell antiserum and staining with polycationic ferritin demonstrated that bacteria were enveloped by extensive EPS. Antibody stabilization permitted a visualization of the EPS which more closely resembled its natural hydrated state. The EPS was seen to completely fill the intercellular spaces in the microcolonies. Both pure and mixed microcolonies were observed to be enclosed by EPS. The presence of these large amounts of EPS indicates that this extracellular layer is important in maintaining the structural integrity of granular sludge.
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页码：481 / 485
页数：5
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