PURIFICATION OF CAENORHABDITIS-ELEGANS DNA TOPOISOMERASE-I

被引：6
|
作者：
PARK, SM [1 ]
KOO, HS [1 ]
机构：
[1] YONSEI UNIV,COLL SCI,DEPT BIOCHEM,SEOUL 120749,SOUTH KOREA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1994年 / 1219卷 / 01期
关键词：
DNA TOPOISOMERASE I; BERENIL; (CAENORHABDITIS ELEGANS);
D O I：
10.1016/0167-4781(94)90245-3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
DNA topoisomerase I was partially purified from Caenorhabditis elegans worms. The enzyme is a 95 kDa polypeptide and its proteolytically degraded form of 70 kDa was also observed. The enzyme removed not only negative but also positive DNA supercoils. The optimum salt concentration for the DNA relaxation activity was 100 mM KCl, and divalent cations were not required but stimulated the activity. The DNA relaxation activity was weakly sensitive to 125 mu M camptothecin but was completely inhibited by 125 mu M berenil.
引用
收藏
页码：47 / 54
页数：8
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