RAPID RESTRICTION MAPPING OF DNA CLONED IN LAMBDA PHAGE VECTORS

被引：258

作者：

RACKWITZ, HR ^{[1
]}

ZEHETNER, G ^{[1
]}

FRISCHAUF, AM ^{[1
]}

LEHRACH, H ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB, MEYERHOFSTR 1, D-6900 HEIDELBERG, FED REP GER

来源：

GENE | 1984年 / 30卷 / 1-3期

关键词：

D O I：

10.1016/0378-1119(84)90120-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A protocol for the rapid restriction mapping of phage .lambda. clones was developed. Partial digestion products are selectively labeled at the right or left cohesive .lambda. DNA termini by hybridization with [32P]oligonucleotides complementary to the single-stranded cos ends. After gel electrophoresis and autoradiography, the restriction map can be directly determined from the ladder of partial digestion products.

引用

页码：195 / 200

页数：6

共 10 条

[1] VARIATION BETWEEN MOUSE MAJOR URINARY PROTEIN GENES ISOLATED FROM A SINGLE INBRED LINE [J].

CLARK, AJ ;

CLISSOLD, PM ;

BISHOP, JO .

GENE, 1982, 18 (03) :221-230

[2]

Davidson N., 1971, The Bacteriophage Lambda, P45

[3] SEPARATION OF VERY LARGE DNA-MOLECULES BY GEL-ELECTROPHORESIS [J].

FANGMAN, WL .

NUCLEIC ACIDS RESEARCH, 1978, 5 (03) :653-665

[4] LAMBDA-REPLACEMENT VECTORS CARRYING POLYLINKER SEQUENCES [J].

FRISCHAUF, AM ;

LEHRACH, H ;

POUSTKA, A ;

MURRAY, N .

JOURNAL OF MOLECULAR BIOLOGY, 1983, 170 (04) :827-842

[5] SELECTIVE ELIMINATION OF EXONUCLEASE ACTIVITY OF DEOXYRIBONUCLEIC ACID POLYMERASE FROM ESCHERICHIA-COLI-B BY LIMITED PROTEOLYSIS [J].

KLENOW, H ;

HENNINGSEN, I .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1970, 65 (01) :168-+

[6] FRACTIONATION OF HIGH-MOLECULAR-WEIGHT RIBONUCLEIC ACID BY POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].

LOENING, UE .

BIOCHEMICAL JOURNAL, 1967, 102 (01) :251-+

[7]

MANIATIS T., 1982, MOL CLONING LABORATO

[8] NUCLEOTIDE-SEQUENCE OF BACTERIOPHAGE-GAMMA DNA [J].

SANGER, F ;

COULSON, AR ;

HONG, GF ;

HILL, DF ;

PETERSEN, GB .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 162 (04) :729-773

[9] SIMPLE METHOD FOR DNA RESTRICTION SITE MAPPING [J].

SMITH, HO ;

BIRNSTIEL, ML .

NUCLEIC ACIDS RESEARCH, 1976, 3 (09) :2387-2398

[10] RAPID BACTERIOPHAGE SEDIMENTATION IN PRESENCE OF POLYETHYLENE GLYCOL AND ITS APPLICATION TO LARGE-SCALE VIRUS PURIFICATION [J].

YAMAMOTO, KR ;

ALBERTS, BM ;

BENZINGE.R ;

LAWHORNE, L ;

TREIBER, G .

VIROLOGY, 1970, 40 (03) :734-+

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