Optimized Protocol for Protein Extraction from the Breast Tissue that is Compatible with Two-Dimensional Gel Electrophoresis

被引：9

作者：

Zakharchenko, Olena ^{[1
,4
]}

Greenwood, Christina ^{[2
]}

Alldridge, Louise ^{[3
]}

Souchelnytskyi, Serhiy ^{[1
]}

机构：

[1] Karolinska Inst, Karolinska Biom Ctr, Dept Oncol Pathol, Canc Translat Res Unit, Stockholm, Sweden

[2] Anglia Ruskin Univ, Helen Rollason Res Lab, Chelmsford, Essex, England

[3] Griffith Univ, Sch Med, Gold Coast, Australia

[4] KBC, CTR Unit, SE-17176 Stockholm, Sweden

来源：

BREAST CANCER-BASIC AND CLINICAL RESEARCH | 2011年 / 5卷

基金：

瑞典研究理事会;

关键词：

2D-GE; breast tissue; sample preparation; SBO4; buffer;

D O I：

10.4137/BCBCR.S6263

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Proteomics is a highly informative approach to analyze cancer-associated transformation in tissues. The main challenge to use a tissue for proteomics studies is the small sample size and difficulties to extract and preserve proteins. The choice of a buffer compatible with proteomics applications is also a challenge. Here we describe a protocol optimized for the most efficient extraction of proteins from the human breast tissue in a buffer compatible with two-dimensional gel electrophoresis (2D-GE). This protocol is based on mechanically assisted disintegration of tissues directly in the 2D-GE buffer. Our method is simple, robust and easy to apply in clinical practice. We demonstrate high quality of separation of proteins prepared according to the reported here protocol.

引用

页码：37 / 42

页数：6

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