IDENTIFICATION OF A PROTEIN THAT PURIFIES WITH THE SCRAPIE PRION

被引：1061

作者：

BOLTON, DC ^{[1
]}

MCKINLEY, MP ^{[1
]}

PRUSINER, SB ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO, DEPT BIOCHEM & BIOPHYS, SAN FRANCISCO, CA 94143 USA

来源：

SCIENCE | 1982年 / 218卷 / 4579期

关键词：

D O I：

10.1126/science.6815801

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Purification of prions from scrapie-infected hamster brain yielded a protein that was not found in a similar fraction from uninfected brain. The protein migrated with an apparent molecular size of 27,000-30,000 daltons in sodium dodecyl sulfate polyacrylamide gels. The resistance of this protein to digestion by proteinase K distinguished it from proteins of similar MW found in normal hamster brain. Initial results suggest that the amount of this protein correlates with the titer of the agent.

引用

页码：1309 / 1311

页数：3

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