IDENTIFICATION OF A PROTEIN THAT PURIFIES WITH THE SCRAPIE PRION

被引：1065

作者：

BOLTON, DC ^{[1
]}

MCKINLEY, MP ^{[1
]}

PRUSINER, SB ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO, DEPT BIOCHEM & BIOPHYS, SAN FRANCISCO, CA 94143 USA

来源：

SCIENCE | 1982年 / 218卷 / 4579期

关键词：

D O I：

10.1126/science.6815801

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Purification of prions from scrapie-infected hamster brain yielded a protein that was not found in a similar fraction from uninfected brain. The protein migrated with an apparent molecular size of 27,000-30,000 daltons in sodium dodecyl sulfate polyacrylamide gels. The resistance of this protein to digestion by proteinase K distinguished it from proteins of similar MW found in normal hamster brain. Initial results suggest that the amount of this protein correlates with the titer of the agent.

引用

页码：1309 / 1311

页数：3

共 26 条

[1] DOES AGENT OF SCRAPIE REPLICATE WITHOUT NUCLEIC ACID [J].

ALPER, T ;

CRAMP, WA ;

HAIG, DA ;

CLARKE, MC .

NATURE, 1967, 214 (5090) :764-&

[2] EXCEPTIONALLY SMALL SIZE OF SCRAPIE AGENT [J].

ALPER, T ;

HAIG, DA ;

CLARKE, MC .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1966, 22 (03) :278-&

[3] LABELING OF PROTEINS TO HIGH SPECIFIC RADIOACTIVITIES BY CONJUGATION TO A I-125-CONTAINING ACYLATING AGENT - APPLICATION TO RADIOIMMUNOASSAY [J].

BOLTON, AE ;

HUNTER, WM .

BIOCHEMICAL JOURNAL, 1973, 133 (03) :529-538

[4]

BOLTON DC, UNPUB

[5] FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS [J].

BONNER, WM ;

LASKEY, RA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01) :83-88

[6]

BURNSTEIN Y, 1974, BIOCHEMISTRY-US, V13, P205

[7] VIROIDS AND PRIONS [J].

DIENER, TO ;

MCKINLEY, MP ;

PRUSINER, SB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1982, 79 (17) :5220-5224

[8]

DURBIN RK, 1982, THESIS U CALIFORNIA

[9] UNCONVENTIONAL VIRUSES AND ORIGIN AND DISAPPEARANCE OF KURU [J].

GAJDUSEK, DC .

SCIENCE, 1977, 197 (4307) :943-960

[10] IONIC PROPERTIES OF AN ESSENTIAL HISTIDINE RESIDUE IN PIG HEART LACTATE-DEHYDROGENASE [J].

HOLBROOK, JJ ;

INGRAM, VA .

BIOCHEMICAL JOURNAL, 1973, 131 (04) :729-738

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