SEQUENCE AND ARRANGEMENT OF 2 GENES OF THE BUTYRATE-SYNTHESIS PATHWAY OF CLOSTRIDIUM-ACETOBUTYLICUM ATCC-824

被引：48
|
作者：
WALTER, KA
NAIR, RV
CARY, JW
BENNETT, GN
PAPOUTSAKIS, ET
机构：
[1] NORTHWESTERN UNIV,DEPT CHEM ENGN,EVANSTON,IL 60208
[2] USDA ARS,SO REG RES CTR,NEW ORLEANS,LA 70179
[3] RICE UNIV,DEPT BIOCHEM & CELL BIOL,HOUSTON,TX 77251
来源：
GENE | 1993年 / 134卷 / 01期
基金：
美国国家科学基金会;
关键词：
RECOMBINANT DNA; PHOSPHOTRANSBUTYRYLASE; BUTYRATE KINASE; PRIMARY METABOLISM; TRANSCRIPTION ANALYSIS;
D O I：
10.1016/0378-1119(93)90182-3
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The genes encoding both Clostridium acetobutylicum ATCC 824 butyrate synthesis pathway enzymes, phosphotransbutyrylase (ptb) and butyrate kinase (buk), were sequenced. The genes are immediately adjacent on the chromosome, with ptb preceding buk. A single transcription start point (tsp) was identified 57 bp upstream from the ptb start codon by primer extension analysis. The ptb and buk genes appear to form an operon. A putative Rho-independent terminator structure was identified 26 bp downstream from buk.
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页码：107 / 111
页数：5
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