IN-VITRO MEASUREMENT OF TRANSLATION ACCURACY OF RIBOSOMES ISOLATED FROM STREPTOMYCIN-RESISTANT MUTANT OF STREPTOMYCES GRANATICOLOR

被引：0

作者：

WEISER, J ^{[1
]}

EHRENBERG, M ^{[1
]}

NAPRSTEK, J ^{[1
]}

机构：

[1] UPPSALA UNIV, BIOMED CTR, S-75124 UPPSALA, SWEDEN

来源：

FOLIA MICROBIOLOGICA | 1994年 / 39卷 / 02期

关键词：

D O I：

10.1007/BF02906807

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Accuracy of activity of ribosome isolated from W-light-induced streptomycin-resistant R-21 mutant of Streptomyces granaticolor was measured in an E. coli-derived system translating poly(U) with a high rate and accuracy. Ribosomes from the R-21 mutant strain were shown to be resistant to streptomycin and about two-fold more accurate than those from the wild type. The mutant strain was found to be resistant to 1000 mg/L streptomycin (Stm) during vegetative growth while it sporulated on agar plates containing only up to 200 mg/L of Stm. The growth rate of the R-21 mutant in complex liquid medium was indistinguishable from that of the wild-type strain.

引用

页码：129 / 132

页数：4

共 15 条

[1] STREPTOMYCIN-RESISTANT, ASPOROGENOUS MUTANT OF BACILLUS-SUBTILIS [J].