INFLUENCE OF MONOCLONAL AND POLYCLONAL PEROXIDASE-SPECIFIC ANTIBODIES ON PEROXIDASE-DEPENDENT COOXIDATION OF 4-IODOPHENOL WITH LUMINOL AND 4-AMINOANTIPYRINE

Peroxidase-dependent cooxidation of 4-iodophenol and 4-aminoantipyrine (AAP), and 4-iodophenol and luminol has been studied The influence of polyclonal and monoclonal antibodies (polyAb and monoAb) to horseradish peroxidase on the kinetics of the reaction was investigated over a wide range of H2O2 concentrations (0.01-10.0 Mm). Three types of MonoAb (2C, 3E, and 9D) in the concentration range 0.05-500 Nm inhibited the cooxidation of AAP and 4-iodophenol at H2O2 concentrations above 1.0 Mm but activated the cooxidation of luminol and 4-iodophenol. Binary and ternary mixtures of monoAb activated the cooxidation of AAP and 4-iodophenol at the same H2O2 concentrations but inhibited or did not affect the cooxidation of luminol and 4-iodophenol. PolyAb activated the cooxidation of AAP and 4-iodophenol more effectively and only slightly activated (or inhibited) the cooxidation of luminol and 4-iodophenol. PolyAb diminished rate constants for the interaction of peroxidase active intermediates, E1 and E2, with 4-iodophenol, AAP, and luminol. Possible mechanisms for the influence of the antibodies on the cooxidation of the substrate pairs are discussed