ANALYSIS OF ECDYSTEROIDS IN THE TREMATODES, SCHISTOSOMA-MANSONI AND FASCIOLA-HEPATICA

被引：0
|
作者：
FOSTER, JM [1 ]
MERCER, JG [1 ]
REES, HH [1 ]
机构：
[1] UNIV LIVERPOOL,DEPT BIOCHEM,LIVERPOOL L69 3BX,ENGLAND
来源：
TROPICAL MEDICINE AND PARASITOLOGY | 1992年 / 43卷 / 04期
基金：
英国惠康基金;
关键词：
D O I：
暂无
中图分类号：
R38 [医学寄生虫学]; Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ; 100103 ;
摘要：
Adult Schistosoma mansoni from experimentally infected mice and Fasciola hepatica recovered from ovine livers post mortem were analyzed for free and conjugated ecdysteroids by radioimmunoassay, high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by capillary gas chromatography/mass spectrometry (selected ion monitoring). Both species contained ecdysone and 20-hydroxyecdysone as free ecdysteroids and as polar conjugates. F. hepatica also contained a polar conjugate of 2-deoxyecdysone. Evidence of apolar ecdysteroid conjugates was only obtained for F. hepatica. The free ecdysteroid-containing fraction of S. mansoni contained small amounts of unidentified immunoreactive material.
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页码：239 / 244
页数：6
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