PHOSPHORYLATED POLYSTYRENE RESINS IN HIGH-PERFORMANCE ION-EXCHANGE CHROMATOGRAPHY

被引：4
|
作者：
LETOURNEUR, D
MIGONNEY, V
MULLER, D
JOZEFOWICZ, M
机构：
[1] LRM, CNRS URA 502, University Paris XIII, 93430 Villetaneuse, Avenue J. B. Clément
来源：
JOURNAL OF CHROMATOGRAPHY | 1992年 / 589卷 / 1-2期
关键词：
D O I：
10.1016/0021-9673(92)80008-I
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Non-porous polystyrene beads were highly substituted with phosphate groups, grafted via spacers on hydroxylated polystyrene. The phosphorylated polystyrene resins exhibit ion-exchange properties that are different to those of the usual stationary phases containing carboxymethyl or sulphopropyl groups. Moreover, the substituted polymers possess high mechanical and chemical stability, hydrophilicity and ion-exchange capacity. Various proteins were fractionated with these phosphorylated resins, packed in high-performance liquid chromatographic columns. The chromatographic separation occurred according to an ion-exchange mechanism; the adsorption of biomolecules was controlled by the pH and ionic strength of the eluent.
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页码：87 / 91
页数：5
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