IDENTIFICATION OF BETA-2-ADRENOCEPTORS ON GUINEA-PIG ALVEOLAR MACROPHAGES USING (-)-3-[I-125]IODOCYANOPINDOLOL

被引：3
作者：
LEURS, R [1 ]
BEUSENBERG, FD [1 ]
BAST, A [1 ]
VANAMSTERDAM, JGC [1 ]
TIMMERMAN, H [1 ]
机构：
[1] ERASMUS UNIV,FAC MED,DEPT PHARMACOL,3000 DR ROTTERDAM,NETHERLANDS
来源：
INFLAMMATION | 1990年 / 14卷 / 04期
关键词：
D O I：
10.1007/BF00914093
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The β-adrenoceptor antagonist (-)-3-[125I]iodocyanopindolol ([125I]ICYP) binds with high affinity and in a saturable way to membranes of guinea pig alveolar macrophages. The equilibrium dissociation constant for [125I]ICYP is 24.3 ± 1.2 pM, and the number of binding sites is 166.3 ± 13.7 fmol/mg protein (N=4, ±SEM). Displacement studies with selective antagonists showed that [125I]ICYP labels β2-adrenoceptors on guinea pig alveolar macrophages. © 1990 Plenum Publishing Corporation.
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页码：421 / 426
页数：6
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