OCCURRENCE OF MULTIPLE LECTINS IN THE HEMOLYMPH OF KURUMA PRAWN PENAEUS-JAPONICUS

被引：11

作者：

MURAMOTO, K ^{[1
]}

MATSUDA, T ^{[1
]}

NAKADA, K ^{[1
]}

KAMIYA, H ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC AGR,SENDAI,MIYAGI 981,JAPAN

来源：

FISHERIES SCIENCE | 1995年 / 61卷 / 01期

关键词：

LECTINS; HEMAGGLUTININS; AGGLUTININS; HEMOLYMPH; KURUMA PRAWN; PENAEUS-JAPONICUS;

D O I：

10.2331/fishsci.61.131

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The hemolymph of kuruma prawn Penaeus japonicus agglutinated horse, sheep, chicken, and human erythrocytes, but not a marine bacterium Vibrio anguillarium. The hemagglutinating activity was dependent on the presence of calcium ions. The higher titer values were obtained by keeping the reaction mixture at 6-degrees-C for 16 h compared to those observed at room temperature. The hemagglutinating activity was inhibited by glycoproteins and simple sugars such as mucin Type I, mucin Type II, fetuin, D-mannosamine, N-acetyl-D-glucosamine, and N-acetylneuraminic acid. The lectins were purified by affinity chromatography on mucin Type II-Sepharose 6B. Succeeding separation by ion-exchange chromatography and gel-filtration revealed that the affinity purified lectins were a mixture of the lectins with different molecular species and also sugar-binding specificity. The two lectins were obtained in electrophoretically pure form; one (Fraction B-1) was a protein of 370kDa consisted of subuntis of 84 kDa and the other (Fraction D, 240 kDa) consisted with subunits of 34 kDa.

引用

页码：131 / 135

页数：5

共 15 条

[11]

TAKAHASHI Y, 1985, B JPN SOC SCI FISH, V51, P721

[12]

TAKAHASHI Y, 1985, B JPN SOC SCI FISH, V51, P1639

[13] PURIFICATION AND CARBOHYDRATE-BINDING SPECIFICITIES OF A BLOOD TYPE-B BINDING LECTIN FROM HEMOLYMPH OF A CRAB (CHARYBDIS-JAPONICA) [J].