COMPARISON OF METHANE HYDROXYLATION ACTIVITY WITH INTACT-CELLS AND CELL-FREE-EXTRACTS OF METHYLOSINUS-TRICHOSPORIUM OB3B

被引：1
|
作者：
SUGIMORI, D [1 ]
OKURA, I [1 ]
机构：
[1] TOKYO INST TECHNOL,DEPT BIOENGN,MIDORI KU,YOKOHAMA,KANAGAWA 227,JAPAN
来源：
JOURNAL OF MOLECULAR CATALYSIS A-CHEMICAL | 1995年 / 97卷 / 03期
关键词：
ACTIVE SITE NUMBER; HYDROXYLATION; METHANE HYDROXYLATION; METHANE MONOOXYGENASE; METHYLOSINUS TRICHOSPORIUM OB3B; TURNOVER NUMBER;
D O I：
10.1016/1381-1169(95)00034-8
中图分类号：
O64 [物理化学（理论化学）、化学物理学];
学科分类号：
070304 ; 081704 ;
摘要：
The number of active sites for methane hydroxylation of Methylosinus trichosporium OB3b was determined by using the inhibition method. The activity per active site of methane hydroxylation with the intact cells and the cell-free extracts were compared. From the turnover numbers of both systems, the reaction mechanism is discussed.
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页码：L135 / L137
页数：3
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