A NEW METHOD FOR MEASURING ANION TRANSFER ACROSS MEMBRANES OF PIED CELL INSIDE-OUT VESICLES

被引：0

作者：

FENG, C ^{[1
]}

GUAN, ZY ^{[1
]}

TU, YP ^{[1
]}

机构：

[1] ACAD SINICA,INST BIOPHYS,NATL KEY LAB BIOMACROMOLEC,BEIJING 100101,PEOPLES R CHINA

来源：

PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS | 1995年 / 22卷 / 02期

关键词：

ERYTHROCYTE MEMBRANE; BAND; 3; PROTEIN; INSIDE-OUT VESICLES;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method for measuring the anion transport activity of Band 3 in the erythrocyte inside-out vesicles (IOVs) was presented. Impermeable IOVs containing 80mmol/L dipicolinic acid (DPA) were obtained by pressing the IOVs through a No. 9 gauge needle in DPA - containing medium Efflux of DPA from the IOVs into Tb3+-containing transport buffer was monitored fluorometrically. The technique is highly sensitive, reproducible and much convenient as well.

引用

页码：154 / 158

页数：5

共 13 条

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