ORAL IMMUNIZATION WITH A RECOMBINANT BACTERIAL-ANTIGEN PRODUCED IN TRANSGENIC PLANTS

被引：464

作者：

HAQ, TA

MASON, HS

CLEMENTS, JD

ARNTZEN, CJ

机构：

[1] TEXAS A&M UNIV, ALBERT B ALKEK INST BIOSCI & TECHNOL, PLANT BIOTECHNOL PROGRAM, HOUSTON, TX 77030 USA

[2] TULANE UNIV, MED CTR, SCH MED, DEPT MICROBIOL & IMMUNOL, NEW ORLEANS, LA 70112 USA

来源：

SCIENCE | 1995年 / 268卷 / 5211期

关键词：

D O I：

10.1126/science.7732379

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The binding subunit of Escherichia coli heat-labile enterotoxin (LT-B) is a highly active oral immunogen. Transgenic tobacco and potato plants were made with the use of genes encoding LT-B or an LT-B fusion protein with a microsomal retention sequence. The plants expressed the foreign peptides, both of which formed oligomers that bound the natural ligand. Mice immunized by gavage produced serum and gut mucosal anti-LT-B immunoglobulins that neutralized the enterotoxin in cell protection assays. Feeding mice fresh transgenic potato tubers also caused oral immunization.

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收藏

页码：714 / 716

页数：3

相关论文

共 28 条

[1]

ARNTZEN CJ, 1994, VACCINES 94 - MODERN APPROACHES TO NEW VACCINES INCLUDING PREVENTION OF AIDS, P339

[2] FILM DETECTION METHOD FOR TRITIUM-LABELED PROTEINS AND NUCLEIC-ACIDS IN POLYACRYLAMIDE GELS [J].

BONNER, WM ;

LASKEY, RA .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1974, 46 (01) :83-88

[3]

CARDENAS L, 1993, INFECT IMMUN, V61, P4629

[4] BIPARTITE SIGNAL SEQUENCE MEDIATES NUCLEAR TRANSLOCATION OF THE PLANT POTYVIRAL NLA PROTEIN [J].

CARRINGTON, JC ;

FREED, DD ;

LEINICKE, AJ .

PLANT CELL, 1991, 3 (09) :953-962

[5] FIELD TRIAL OF ORAL CHOLERA VACCINES IN BANGLADESH - RESULTS FROM 3-YEAR FOLLOW-UP [J].

CLEMENS, JD ;

SACK, DA ;

HARRIS, JR ;

VANLOON, F ;

CHAKRABORTY, J ;

AHMED, F ;

RAO, MR ;

KHAN, MR ;

YUNUS, M ;

HUDA, N ;

STANTON, BF ;

KAY, BA ;

WALTER, S ;

EECKELS, R ;

SVENNERHOLM, AM ;

HOLMGREN, J .

LANCET, 1990, 335 (8684) :270-273

[6] IMMUNOLOGICAL CROSS-REACTIVITY BETWEEN A HEAT-LABILE ENTEROTOXIN(S) OF ESCHERICHIA-COLI AND SUBUNITS OF VIBRIO-CHOLERAE ENTEROTOXIN [J].

CLEMENTS, JD ;

FINKELSTEIN, RA .

INFECTION AND IMMUNITY, 1978, 21 (03) :1036-1039

[7] CONSTRUCTION OF A POTENTIAL LIVE ORAL BIVALENT VACCINE FOR TYPHOID-FEVER AND CHOLERA-ESCHERICHIA-COLI-RELATED DIARRHEAS [J].

CLEMENTS, JD ;

ELMORSHIDY, S .

INFECTION AND IMMUNITY, 1984, 46 (02) :564-569

[8] ORAL VACCINATION - IDENTIFICATION OF CLASSES OF PROTEINS THAT PROVOKE AN IMMUNE-RESPONSE UPON ORAL-FEEDING [J].

DEAIZPURUA, HJ ;

RUSSELLJONES, GJ .

JOURNAL OF EXPERIMENTAL MEDICINE, 1988, 167 (02) :440-451

[9] COORDINATED ASSEMBLY OF MULTISUBUNIT PROTEINS - OLIGOMERIZATION OF BACTERIAL ENTEROTOXINS INVIVO AND INVITRO [J].

HARDY, SJS ;

HOLMGREN, J ;

JOHANSSON, S ;

SANCHEZ, J ;

HIRST, TR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (19) :7109-7113

[10] CONFORMATION OF PROTEIN SECRETED ACROSS BACTERIAL OUTER MEMBRANES - A STUDY OF ENTEROTOXIN TRANSLOCATION FROM VIBRIO-CHOLERAE [J].

HIRST, TR ;

HOLMGREN, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) :7418-7422