A PCR-BASED SEX-DETERMINATION ASSAY IN CATTLE BASED ON THE BOVINE AMELOGENIN LOCUS

被引：196

作者：

ENNIS, S

GALLAGHER, TF

机构：

[1] NATL UNIV IRELAND UNIV COLL DUBLIN, FAC MED VET, DEPT ANIM HUSB, DUBLIN, IRELAND

[2] NATL UNIV IRELAND UNIV COLL DUBLIN, FAC SCI, DEPT BOT, DUBLIN, IRELAND

来源：

ANIMAL GENETICS | 1994年 / 25卷 / 06期

关键词：

AMELOGENIN; PCR; SEX DETERMINATION; BOVINE EMBRYO; BIOPSIES;

D O I：

10.1111/j.1365-2052.1994.tb00533.x

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

A method for determining the sex of bovine embryos has been established. Primers for a portion of the bovine amelogenin locus (AMX/Y) were used to amplify DNA present in either 0.1 mu l of blood or biopsies taken from 6-7-day-old embryos. The primers amplify a 280 bp band in females and a 280 and 217 bp bands in males. The method is rapid, does not require prior purification of DNA and contains an internal control which detects PCR failure.

引用

页码：425 / 427

页数：3

共 6 条

[1] AMPLIFICATION OF THE ZFY AND ZFX GENES FOR SEX IDENTIFICATION IN HUMANS, CATTLE, SHEEP AND GOATS [J].