A PCR-BASED SEX-DETERMINATION ASSAY IN CATTLE BASED ON THE BOVINE AMELOGENIN LOCUS

被引:196
作者
ENNIS, S
GALLAGHER, TF
机构
[1] NATL UNIV IRELAND UNIV COLL DUBLIN, FAC MED VET, DEPT ANIM HUSB, DUBLIN, IRELAND
[2] NATL UNIV IRELAND UNIV COLL DUBLIN, FAC SCI, DEPT BOT, DUBLIN, IRELAND
关键词
AMELOGENIN; PCR; SEX DETERMINATION; BOVINE EMBRYO; BIOPSIES;
D O I
10.1111/j.1365-2052.1994.tb00533.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
A method for determining the sex of bovine embryos has been established. Primers for a portion of the bovine amelogenin locus (AMX/Y) were used to amplify DNA present in either 0.1 mu l of blood or biopsies taken from 6-7-day-old embryos. The primers amplify a 280 bp band in females and a 280 and 217 bp bands in males. The method is rapid, does not require prior purification of DNA and contains an internal control which detects PCR failure.
引用
收藏
页码:425 / 427
页数:3
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