A SIMPLE SYNTHESIS OF OCTYL 3,6-DI-O-(ALPHA-D-MANNOPYRANOSYL)-BETA-D-MANNOPYRANOSIDE AND ITS USE AS AN ACCEPTOR FOR THE ASSAY OF N-ACETYLGLUCOSAMINYLTRANSFERASE-I ACTIVITY

被引：48
|
作者：
KAUR, KJ [1 ]
HINDSGAUL, O [1 ]
机构：
[1] UNIV ALBERTA,DEPT CHEM,EDMONTON T6G 2G2,ALBERTA,CANADA
来源：
GLYCOCONJUGATE JOURNAL | 1991年 / 8卷 / 02期
关键词：
N-ACETYLGLUCOSAMINYLTRANSFERASE-I; GLYCOSYLTRANSFERASE ASSAY; CORE-TRIMANNOSIDE; OLIGOSACCHARIDE SYNTHESIS;
D O I：
10.1007/BF00731017
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A simple synthesis of octyl 3,6-di-O-(alpha-D-mannopyranosyl)-beta-D-mannopyranoside is described. The key features of the synthetic scheme are the formation of the beta-mannosidic linkage by 1-O-alkylation of 2,3,4,6-tetra-O-acetyl-alpha,beta-D-mannopyranose with octyl iodide and glycosylation of unprotected octyl beta-D-mannopyranoside using limiting acetobromomannose. The trisaccharide is shown to be an acceptor for N-acetylglucosaminyltransferase-I with a K(M) of 585-mu-M.
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页码：90 / 94
页数：5
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