PRIMARY CULTURES OF GUINEA-PIG GALLBLADDER EPITHELIAL-CELLS SUITABLE FOR PATCH-CLAMP STUDIES

被引：6
|
作者：
OBRIEN, JA [1 ]
JAMES, PS [1 ]
TORRE, M [1 ]
机构：
[1] DIPARTIMENTO FISIOL & BIOCHIM GEN ELETTROFISIOL,MILAN,ITALY
来源：
EXPERIMENTAL PHYSIOLOGY | 1991年 / 76卷 / 05期
关键词：
D O I：
10.1113/expphysiol.1991.sp003546
中图分类号：
Q4 [生理学];
学科分类号：
071003 ;
摘要：
Cells derived from explants of guinea-pig gall-bladder maintained in culture for up to 14 days, exhibit a network of cytoskeletal filaments that are labelled by an anti-cytokeratin antibody, but not by an anti-desmin antibody, suggesting that they are of epithelial origin. These cells proved suitable for patch-clamp studies and by using this method channels selective for K+ and Cl-were observed.
引用
收藏
页码：807 / 810
页数：4
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