REGIONAL QUANTIFICATION OF DOPAMINE TRANSPORTER MESSENGER-RNA IN RAT-BRAIN USING A RIBONUCLEASE PROTECTION ASSAY

被引：9
作者：
RICHTAND, NM
KELSOE, JR
SEGAL, DS
KUCZENSKI, R
机构：
[1] SAN DIEGO VET AFFAIRS MED CTR, DEPT PSYCHIAT, LA JOLLA, CA 92161 USA
[2] UNIV CALIF SAN DIEGO, DEPT PSYCHIAT, LA JOLLA, CA 92093 USA
来源：
NEUROSCIENCE LETTERS | 1995年 / 200卷 / 02期
关键词：
DOPAMINE TRANSPORTER; MESSENGER-RNA; SUBSTANTIA NIGRA; HYPOTHALAMUS; RIBONUCLEASE PROTECTION ASSAY;
D O I：
10.1016/0304-3940(95)12096-M
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We describe the regional distribution of dopamine transporter (DAT) mRNA in selected regions of rat brain using a highly sensitive and specific nuclease protection assay. This method determines the absolute quantity of mRNA expressed in the brain regions surveyed. DAT mRNA level varied widely between brain regions, and was detected only in cell body regions of the major dopaminergic pathways. Highest expression was seen in substantia nigra/ventral tegmentum (SN/VTA). Lower but detectable expression of a protected mRNA of the expected size was observed within hypothalamus. Expression could not be detected by this method in other brain regions studied. Our results indicate that this method is sufficiently sensitive to allow study of mRNA expression in individual animals.
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收藏
页码：73 / 76
页数：4
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