ADSORPTION DYNAMICS OF A PHOSPHOLIPASE A(2) ONTO A MERCURY-ELECTRODE SURFACE

被引：6

作者：

CHEN, SW ^{[1
]}

ABRUNA, HD ^{[1
]}

机构：

[1] CORNELL UNIV, BAKER LAB, DEPT CHEM, ITHACA, NY 14853 USA

来源：

JOURNAL OF PHYSICAL CHEMISTRY | 1995年 / 99卷 / 47期

关键词：

D O I：

10.1021/j100047a030

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Rapid cyclic voltammetry has been employed to investigate the electrochemical behavior of porcine pancreatic phospholipase A(2) (PLA(2)) adsorbed onto a hanging mercury drop electrode surface. The electrochemical response of PLA(2) exhibits a pair of voltammetric peaks which are ascribed to the reduction/oxidation of cystine/cysteine residues within the enzyme strongly adsorbed on mercury surfaces over the pH range of 0.77-8.50. Based on theoretical fits to the data, the adsorption process, under all experimental conditions, is found to be kinetically controlled. In addition, the adsorption appears to be consistent with a Frumkin adsorption isotherm with a small repulsive interaction. Both pH and temperature have a dramatic effect on the adsorption behavior in terms of the adsorption rate, equilibrium coverage, and molecular conformation.

引用

页码：17235 / 17243

页数：9

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