2 HELICES PLUS A LINKER - A SMALL MODEL SUBSTRATE FOR EUKARYOTIC RNASE-P

被引：32

作者：

CARRARA, G

CALANDRA, P

FRUSCOLONI, P

TOCCHINIVALENTINI, GP

机构：

[1] CNR,IST BIOL CELLULARE,I-00137 ROME,ITALY

[2] ENICHEM SPA,BIOTECNOL LAB,I-00015 MONTEROTONDO,ITALY

[3] UNIV CHICAGO,DEPT BIOCHEM & MOLEC BIOL,CHICAGO,IL 60637

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 07期

关键词：

XENOPUS LAEVIS; TRNAPROCESSING;

D O I：

10.1073/pnas.92.7.2627

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Using precursor tRNA molecules to study RNA-protein interactions, we have identified an RNA motif recognized by eukaryotic RNase P (EC 3.1.26.5). Analysis of circularly permuted precursors indicates that interruptions in the sugar-phosphate backbone are not tolerated in the acceptor stem, in the T stem-loop, or between residues A-9 and G-10. Prokaryotic RNase P will function with a minihelix consisting of the acceptor stem connected directly to the T stem-loop. Eukaryotic RNase P cannot use such a minimal substrate unless a linker sequence is added in the gap where the D stem and anticodon stem-loop were deleted.

引用

页码：2627 / 2631

页数：5

共 24 条

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