2 HELICES PLUS A LINKER - A SMALL MODEL SUBSTRATE FOR EUKARYOTIC RNASE-P

被引：32
作者：
CARRARA, G
CALANDRA, P
FRUSCOLONI, P
TOCCHINIVALENTINI, GP
机构：
[1] CNR,IST BIOL CELLULARE,I-00137 ROME,ITALY
[2] ENICHEM SPA,BIOTECNOL LAB,I-00015 MONTEROTONDO,ITALY
[3] UNIV CHICAGO,DEPT BIOCHEM & MOLEC BIOL,CHICAGO,IL 60637
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1995年 / 92卷 / 07期
关键词：
XENOPUS LAEVIS; TRNAPROCESSING;
D O I：
10.1073/pnas.92.7.2627
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Using precursor tRNA molecules to study RNA-protein interactions, we have identified an RNA motif recognized by eukaryotic RNase P (EC 3.1.26.5). Analysis of circularly permuted precursors indicates that interruptions in the sugar-phosphate backbone are not tolerated in the acceptor stem, in the T stem-loop, or between residues A-9 and G-10. Prokaryotic RNase P will function with a minihelix consisting of the acceptor stem connected directly to the T stem-loop. Eukaryotic RNase P cannot use such a minimal substrate unless a linker sequence is added in the gap where the D stem and anticodon stem-loop were deleted.
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页码：2627 / 2631
页数：5
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