TIME-RESOLVED POLARIZED FLUORESCENCE STUDIES THE TEMPERATURE ADAPTATION IN BACILLUS-SUBTILIS USING DPH AND TMA-DPH FLUORESCENT-PROBES

被引：34

作者：

HERMAN, P ^{[1
]}

KONOPASEK, I ^{[1
]}

PLASEK, J ^{[1
]}

SVOBODOVA, J ^{[1
]}

机构：

[1] CHARLES UNIV,FAC SCI,DEPT MICROBIOL,CS-12844 PRAGUE 2,CZECH REPUBLIC

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1994年 / 1190卷 / 01期

关键词：

ANISOTROPY; MEMBRANE; LIPID; ORDER;

D O I：

10.1016/0005-2736(94)90028-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The validity of the concept of homeoviscous adaptation was tested for bacteria Bacillus subtilis. The Bacillus subtilis grown at 20 degrees C (referred to as Bs20) exhibit a considerable increase of branched anteiso-C-15, the major fatty acid component of membrane lipids, relative to membranes grown at 40 degrees C (Bs40). The time-resolved fluorescence depolarization of 1,6-diphenyl-1,3,5-hexatriene (DPH) and 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH) showed that these changes in the lipid composition are accompanied by changes in a mean lipid order. In particular, the DPH order parameters (P-2) and (P-4) measured in Bs20 membranes at 18 degrees C and in Bs40 membranes at 45 degrees C, respectively, tend to be equal. This effect was less pronounced for TMA-DPH. Our observations suggest that a physical parallel to the changes of lipid composition is the maintenance of an optimal lipid order in the hydrophobic core of the cytoplasmic membranes. It can be interpreted as a tendency of Bacillus subtilis to keep the lateral pressure in its membranes at an optimal value, independent of the temperature of cultivation.

引用

页码：1 / 8

页数：8

共 26 条

[11] MEMBRANE FLUIDITY AS DETECTED BY DIPHENYLHEXATRIENE PROBES [J].

LENTZ, BR .

CHEMISTRY AND PHYSICS OF LIPIDS, 1989, 50 (3-4) :171-190

[12]

LOWRY OH, 1951, J BIOL CHEM, V193, P2630

[13] EFFECT OF TEMPERATURE ON COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI [J].

MARR, AG ;

INGRAHAM, JL .

JOURNAL OF BACTERIOLOGY, 1962, 84 (06) :1260-&

[14]

McElhaney R.N., 1984, MEMBRANE FLUIDITY, P249

[15] THEORETICAL-STUDY OF PROTEIN-LIPID INTERACTIONS IN BILAYER MEMBRANES [J].

OWICKI, JC ;

SPRINGGATE, MW ;

MCCONNELL, HM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (04) :1616-1619

[16]

ROTTEM S, 1978, EUR J BIOCHEM, V85, P450

[17]

SCHREIER S, 1978, BIOCHIM BIOPHYS ACTA, V515, P395, DOI DOI 10.1016/0304-4157(78)90011-4

[18] DYNAMIC STRUCTURE OF FATTY ACYL CHAINS IN A PHOSPHOLIPID BILAYER MEASURED BY DEUTERIUM MAGNETIC-RESONANCE [J].

SEELIG, A ;

SEELIG, J .

BIOCHEMISTRY, 1974, 13 (23) :4839-4845

[19]

SEELIG J, 1978, BIOCHEMISTRY-US, V16, P45

[20] HOMEOVISCOUS ADAPTATION - HOMEOSTATIC PROCESS THAT REGULATES VISCOSITY OF MEMBRANE LIPIDS IN ESCHERICHIA-COLI [J].

SINENSKY, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1974, 71 (02) :522-525

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