RADIOIMMUNOASSAY FOR INSULIN-LIKE GROWTH FACTOR-I IN BOVINE-MILK

被引：12
作者：
ZHAO, X [1 ]
GROENEWEGEN, PP [1 ]
MCBRIDE, BW [1 ]
BURTON, JH [1 ]
ELSASSER, TH [1 ]
机构：
[1] USDA ARS, BELTSVILLE AGR RES CTR, RUMINANT NUTR LAB, BELTSVILLE, MD 20705 USA
来源：
CANADIAN JOURNAL OF ANIMAL SCIENCE | 1991年 / 71卷 / 03期
关键词：
MILK; IGF-I; RADIOIMMUNOASSAY; COWS;
D O I：
10.4141/cjas91-082
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
This experiment was designed (1) to study the effectiveness of acid-ethanol extraction and gly-gly HC1 incubation to dissociate the IGF-binding protein complex before IGF-I RIA measurements in bovine milk, and (2) to determine the influence of freezing/thawing and defatting of milk samples on IGF-I concentration of milk. Acidification of milk with acid-ethanol (acid-ETOH) liberated more IGF-I from its binding proteins than with gly-gly HC1. Milk samples obtained from three control cows and three rbST-treated cows (41-44 wk postpartum) were treated with acid-ethanol and assayed by a radio-immunoassay. IGF-I concentrations in the milk were 3.30 +/- 1.40 for the control cows and 2.77 +/- 1.36 ng mL-1 for the rbST-treated cows. Fat removal before or after freezing did not affect milk IGF-I measurements. The milk concentration of IGF-I after freezing/thawing twice was not different from that after freezing/thawing once. The radioimmunoassay resulting from this study should provide a tool for monitoring IGF-I concentrations in milk throughout the entire lactation.
引用
收藏
页码：669 / 674
页数：6
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